CatalogueNumber: | AN00009 |
ProductName: | anti-6*His,His-Tagantibody |
Form: | liquid |
Host: | Mouse |
Clonity: | monoclonal |
Isotope: | IgG2b |
Storage: | PBSwith0.02%sodiumazideand50%glycerolpH7.3,-20â&BDquo;ƒfor24months(Avoidrepeatedfreeze/thawcycles.) |
Background: | ProteintagsareproteinorpeptidesequenceslocatedeitherontheC-orN-terminalofthetargetprotein,whichfacilitatesoneorseveralofthefollowingcharacteristics:solubility,detection,purification,localizationandexpression.Histagisoftenusedforaffinitypurificationandbindingassays.ExpressedHis-taggedproteinscanbepurifiedanddetectedeasilybecausethestringofhistidineresiduesbindstoseveraltypesofimmobilizedmetalions,includingnickel,cobaltandcopper,underspecificbufferconditions.TheHistagantibodyisausefultoolformonitoringoftheHis-taggedproteins,andrecognizesHis-tagsplacedatN-terminal,C-terminal,andinternalregionsoffusionproteinsexpressedinbacteria,insect,andmammaliancells. |
Synonyms: | 6*His,6*His,HisTag,6histag,6XHis,HHHHHH,HIS,HisTag,HISTIDINETAGGED |
Specificity: | Recombinantprotein |
TestedApplication: | ELISA,WB |
RecommendedDilution: | WB:1:200-1:1000 |
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